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active chk2 enzyme 14-347  (Millipore)


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  • 90

    Structured Review

    Millipore active chk2 enzyme 14-347
    Active Chk2 Enzyme 14 347, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/active+chk2+enzyme+14-347/chk2+inhibitor+ii/us11123326-152-31-34
    Average 90 stars, based on 1 article reviews
    active chk2 enzyme 14-347 - by Bioz Stars, 2026-09
    90/100 stars

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    Serial Dilution:

    Article Title: CHK1/2 inhibitors and irinotecan for use in the treatment of rhabdomyosarcoma
    Article Snippet: .. Final reaction conditions are 50 mM HEPES pH 7.5, 0.005% (v/v) TritonTM X-100, 2 mM DTT, 2 mM MgCl2, 104 nM fluorescein-PKC substrate (Invitrogen, cat #PV3506), 30 μM ATP, 2.5 nM active CHK2 enzyme (Millipore, cat #14-347), 4% (v/v) DMSO and serial dilution of the compound of Example 2 (1:3 serial dilution, starting at 20 μM, 10 points). .. Following ATP addition, the reactions are incubated at room temperature for 75 minutes, and then terminated with the addition of 25 μL of TR-FRET dilution buffer (Invitrogen #PV3574) containing 10 mM EDTA and 2.1 nM Tb-pSer antibody (Invitrogen, cat #PV3574).

    Article Title: 5-(5-(2-(3-aminopropoxy)-6-methoxyphenyl)-1H-pyrazol-3-ylamino)pyrazine-2-carbonitrile, pharmaceutically acceptable salts thereof, or solvate of salts
    Article Snippet: .. Final reaction conditions are 50 mM HEPES pH 7.5, 0.005% (v/v) TRITONTM X-100, 2 mM DTT, 2 mM MgCl2, 104 nM fluorescein-PKC substrate (Invitrogen, cat # PV3506), 30 μM ATP, 2.5 nM active Chk2 enzyme (Millipore, cat #14-347), 4% (v/v) DMSO and serial dilution of the compound of Example 2 (1:3 serial dilution, starting at 20 μM, 10 points). .. Following ATP addition, the reactions are incubated at room temperature for 75 minutes, and then terminated with the addition of 25 μL of TR-FRET dilution buffer (Invitrogen #PV3574) containing 10 mM EDTA and 2.1 nM Tb-pSer antibody (Invitrogen, cat #PV3574).



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    90
    Millipore active chk2 enzyme 14-347
    Active Chk2 Enzyme 14 347, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/active+chk2+enzyme+14-347/chk2+inhibitor+ii/us11123326-152-31-34
    Average 90 stars, based on 1 article reviews
    active chk2 enzyme 14-347 - by Bioz Stars, 2026-09
    90/100 stars
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